Nearly 100 years ago, a seemingly simple discovery revolutionized the microscope. The introduction of phase contrast, which garnered a Nobel Prize in 1953, brought into clear view structures inside ...

Typically in optical microscopy, the signal-to-noise ratio (SNR) is calculated to quantify the image contrast which indicates how well different regions of interest in the image can be differentiated.

Light-sheet fluorescence microscopy (LSFM) has allowed for faster three-dimensional imaging of biological samples with lower photo-bleaching and photo-toxicity than confocal microscopy 1. This is ...

Researchers have introduced a solution to the problem of light-sheet fluorescence microscopy: novel illumination beams designed based on deep learning using a trainable phase mask. Their study ...

A computer-assisted design schematic showing how an electron beam is routed within a microscope. After going through the sample, it crosses a bright cyan beam representing the laser beam. A schematic ...

(A) When a traditional Gaussian beam is used, diffraction causes the illumination beam to widen at the edges. (B) Joint optimization scheme. A network predicts the positions of beads in the focal ...