There are a variety of uses for affinity chromatography purification techniques. They are being increasingly used to separate pharmaceutical and biological samples nowadays. Here are several ways that ...

Affinity chromatography remains one of the main methods for purifying antibodies, enabling high specificity and yield. Whether working with recombinant monoclonal antibodies or polyclonal antibodies ...

Synthetic dyes can act as alternative ligands for proteins and can be used as part of a protein purification protocol for chromatography. Chromatography is the separation of compounds from a mixture.

With new upstream bioprocessing technologies continuing to increase yield and product quantities, the pressure for process improvement has fallen firmly on the downstream purification stage. The ...

The protocol describes a method for capture of secreted hexahistidine-tagged proteins using expanded-bed adsorption immobilized-metal affinity chromatography. The starting material for the procedure ...

Fc receptor based affinity chromatography is a new emerging field of Fc functionality analytics. FcRn affinity columns separate antibody species that differ in their affinity to FcRn using conditions ...

Researchers at the University of Alabama at Birmingham (UAB) have developed a one-step affinity chromatography method for protein purification that they claim is up to 100 times better than existing ...

Purification by affinity chromatography on immobilized calmodulin is suitable for recombinant proteins expressed in bacteria because endogenous bacterial calmodulin-binding proteins are unknown.

From sample preparation to real-time analyses, scientists embarking on protein research journeys need to equip themselves with technical knowledge. A series of rodent experiments showed that even with ...